Secreted Left Atrial P-Selectin in Mitral Stenosis after PBMV: When to Measure
AbstractThe glycoprotein P-selectin, a membrane component of cell storage granules, is rapidly translocated from ?-granules of platelets and the Weibel- Palade bodies of endothelial cells to the cell surface following an inflammatory process or other stimulations. P-selectin is a cell adhesion molecule of activated platelets and endothelial cells of interest because of its role in modulating interactions between blood cells and the endothelium, and also because of the possible use of its soluble form in plasma as a predictor of adverse cardiovascular events.1
In endothelial cells, within minutes of its stimulation in vitro by inflammatory mediators, such as histamine, thrombin, or phorbol esters, or hypoxia, Weibel–Palade bodies are mobilized and their von Willebrand factor are degranulated. At the same time, P-selectin is also expressed at the surface as quick astwo minutes after stimulation. However, this expressionis short-lived, reaching its peak after only 10 minutes, declining back to baseline levels after 3 hours. Additional synthesis of P-selectin is brought about within 2 hours by cytokines, such as interleukin-1 (IL-1), tumor necrosis factor-? (TNF-?), and by thrombin, lipopolysaccharide or oxygen radicals. Immunofluorescence and confocal laser cytometry are usually used to measure the translocation upon this activation.1
2. McEver RP. Adhesive interactions of leukocytes, platelets, and thevessel wall during hemostasis and inflammation. ThrombHaemost2001;86:746–56.
3. Banu N, Avraham S, Avraham HK. P-selectin, and not E-selectin,negatively regulates murine megakaryocytopoiesis. J Immunol2002;169:4579–85.
4. Topaloglu S, Aras D, Ergun K, Geyik B, Ayaz S, Cay S, Cagirci G, Tufekcioglu O, Korkmaz S. Plasma level of soluble P-selectin in patients with rheumatic mitral stenosis and sinus rhythm undergoing percutaneous mitral balloon valvuloplasty. J Thromb and Thrombolysis 2007; 23: 199-204.
5. Hasan-Ali H, Mosad E. Changes in platelet, coagulation, and fibrinolytic activities in mitral stenosis after percutaneous mitral valvotomy: role of hemodynamic change and systemic inflammation. ClinApplThrombHemost 2014 may 19 pii-1076029614533144. [Epub ahead of print].
PDF downloads: 84
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).