The Effect Of Red Rosella Tea (Hibiscus sabdariffaLinn) on the Inhibition of NF-?B Activation, TNF-aand ICAM-1- Protein Expressions Following Treatment with Ox-LDL in HUVECs
Abstract
Background. Atherosclerosis disease has been a global threat. Ox-LDL was one of major source of atherogenesis process, through the ROS formation which would then activate NF-KB and increase the expression of TNF-a and ICAM-1, leading to the occurrence of inflammation. Recent studies indicate that red Rosella tea is a tropical plant containing antioxidant (protocatechuic acid and antocyanin) and ascorbate acid. This tea is widely distributed in Indonesia. The mechanism of action of this tea on the inhibition of atherogenesis has not been largely known. This study aim to investigate the effect and mechanism of the red Rosella tea on the expression of NF-KB, TNF-aand ICAM-1.Methods and results.Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without Ox-LDL as negative control; 2) HUVECs culture with Ox-LDL as positive control; and 3 groups given various dosages of the red Rosella tea (0.001 mg/ml, 0.005 mg/ml and 0.01 mg/ml) and Ox-LDL. OxLDL (40 mg/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking dyslipidemia condition. The Red Rosella tea were given 2 h before Ox-LDL treatment. The NF-KB activaton, the protein expressions of TNF-aand ICAM-1 are detected by immunohistochemistry.
Treatment with red rosella tea on Ox-LDL-treated HUVECs resulted in prevention of NF-KB activation which in turn inhibited the increased of TNF-aand ICAM-1 protein expressions. Spearman‘s correlation analysis showed that there is negative correlations between dosages of red tea with NF-KB activation, TNF-aand ICAM-1 protein expressions (p=0.01, Correlation Coeff= -1).
Conclusions. This study indicates the benefit of red Rosella tea on the prevention and medication for atherosclerosis disease.
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Collins, Tucker., Cybulsky, Myron I. NF-KB : Pitoval Mediator Or Innocent Bystander In Atherogenesis ?. The Jounal Of Clinical Investigation, 2001; 107(3):255-263.
Ross, Russell. Atherosclerosis-An Inflammatory Disease. N. Eng J Med ,1999; 340:115-126
Chang-che chew, et al. Hibiscus Sabdariffa Extract Inhibits The Development Atherosclerosis In Cholesterol-Fed Rabbit. Journal Of Agricultural And Food Chemistry, 2003.
Tseng, T.H., et al. Hibiscus Protocatechuic Acid Protecct Against Oxidative Damage Induced By Tert. Butylhydroperoxide In Rat Primary Hepatocytes. Chem Biol Interact. 1996; 101(2):137-148
Ranzio B. Anti Inflamatory Activities Of Polyphenol-Review : Polyphenol As Anti Inflamatory Agents. Journal Of Naturopathic Medicine 2002; 9:44-50
Ho, Emily. The Virtual Free Radical School : NF-KB- What Is It And What‘S The Deal With Radicals?. Linus Pauling Institute Scintist, Departement Of Nutrition & Food Management, Oregon State University, 2002.
Stache et al. Inhibition Of TNF-Alpha Induced Cell Death In Human Umbilical Vein Endothelial Cells And Jurkat Cell By Protocatechuic Acid. NCBI, Med. Biol. Eng. Comput 2002; 40(6):689-703.
Lee Mj , et al. Hibiscus Protocatechuic Acid Or Esculetin Can Inhibit Oxidative LDL Induced By Either Copper Ion Or Nitric Oxide Donor. Journal Agriculture Food Chemical 2002; 50(7):2130-2136
Farombi, Olatunde E. Review : African Indigenous Plants With Chemotherapeutic Potentials And Biotechnological Approach To The Production Of Bioactive Prophylactic Agent. African Journal Of Biotechnology 2003; 2(12):662-671.
Tseng, T.H., et al. Protective Effect Of Dried Flawer Extract Of Hibiscus Sabdariffa Linn Againts Oxidative Stress In Rat Primery Hepatocytes. Food Chem Toxicol 1997; 35(12):1159-1164.
Jonadet, M, et al. In Vitro Enzyme Inhibitory And In Vivo Cardioprotective Activities Of Hibiscus (Hibiscus Sabdariffa Linn).Journal Pharmacology. Belg 1990;45 (2):120-124
Ali, BH., Mousa HM., El Mougys. The Effect Of Water Extract And Anthocyanins Of Hibiscus Sabdariffa Linn On Paracetamol-Induced Hepatotoxicity In Rats. http://www.google.com, 2003.
Wolinnski, Ira., Hickson, James F. CRC Series In Modern Nutrition : Hanbook Of Nutraceutical And Functional Foods. CRC Press, LLC, Florida, 2000.
Collins, Tucker., Cybulsky, Myron I. NF-KB : Pitoval Mediator Or Innocent Bystander In Atherogenesis ?. The Jounal Of Clinical Investigation, 2001; 107(3):255-263.
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How to Cite
Sarbini, D., Sargowo, D., & Rohman, M. (1). The Effect Of Red Rosella Tea (Hibiscus sabdariffaLinn) on the Inhibition of NF-?B Activation, TNF-aand ICAM-1- Protein Expressions Following Treatment with Ox-LDL in HUVECs. Indonesian Journal of Cardiology, 28(2), 133-141. https://doi.org/10.30701/ijc.v28i2.257
Section
Clinical Research
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