Ratio of EPC and CEC as Endotel Dysfunction Predictor in High Risk Group with Farmingham Risk Score 10 Years Category
Abstract
Research background. Circulating Endothelial Cells (CEC) is a reflection of endothelial damage or endothelial stress, increasing of CEC amount depend on endothelial mechanism, edothelial adhesivity damage and cellular apoptosis as a result of decreasing sitoskleleton function. If higher exposure affects the increasing of CEC amount,VEGF other growth factor mediators will be reflected as endothelial stress manifestation which roles in the increasing of re-population and Endothelial Progenitor Cells (EPC) differentiation. Endothelial Progenitor Cells is a mononuclear cell (a part of stem cell) that could change to be mature endothel and roles in re-edothelialisation and neovascularisation. This research aimed to investigate the ratio of EPC : CEC in risk group through Framingham Risk Score (FRS) 10 years approach as endothelial dysfunction predictor.Research method and result. There were 55 research subjects whom taken by FRS scoring and devided into some risk groups and two control groups. They were control group I (health) and control group II (sick). Base blood was taken to every each of them to analyze their EPC and CEC with Flowcytometry. EPC was analyzed by CD34 Per CP Santa Cruz SC-19621 and CD 133 FITC (fluorescein isothiocyanate) Bioss bs-0395R-FITCmarker.WhileCEC was analyzed CD45 FITC Biolegend 202205 dan CD 146 PE Biolegend 134704 marker. Result showed, there was significant ratio differences of EPC : CEC in those six groups which was proven by p-value 0.032< ? (0.05). The higher ratio was in high risk group (139.06).
Conclusion. Research showed that EPC amount was increase related to the increasing of high risk level according to FRS 10 years, but its increasing did not followed by its ability to homing in injury area as role part in re-endothelialisation process. It found that EPC amount was higher in high risk group than in low risk group.
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References
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Chambliss KL, Shaul PW. Estrogen modulation of endotelial nitric oxide synthase. Endocr Rev 2002;23:665–686.
Boo YC, Hwang J, Sykes M, : Shear stress stimulates phosphorylation of ENOS at ser(635) by a protein kinase A-dependent mechanism. Am J Physiol Heart CircPhysiol 2002;283:H1819–H1828.
Ziegler T, Silacci P, Harrison VJ, : Nitric oxide synthase expression in endotelial cells exposed to mechanical forces. Hypertension 1998;32:351–355.
Rabelink, T. J. : Nat. Rev. Nephrol. 6, 404–414 (2010) Endothelial activation and circulating markers of endothelial activation in kidney disease
John Bayliss Feb 2001., Wilson PWF, : Prediction of coronary heart disease using risk factor categories. Circulation 1998;97:1837-47
Men- Women Estimate of 10-Year Risk for Men (Framingham Point Scores) and ATP III Guidelines At-A-Glance Quick Desk Reference National Institutes of Health National Heart, Lung, and Blood Institute NIH Publication No. 01-3305 May 2001, U.S. Department Of Health And Human Services
Chia Y C, Review of tools of cardiovascular disease risk stratification: interpretation, customisation and application in clinical practice, Singapore Med J 2011; 52(2): 116-123
Bonetti PO, Lerman LO, Lerman A. Endotelial dysfunction: a marker of atherosclerotic risk. Arterioscler ThrombVasc Biol 2003;23:168–175.
Caroline Schmidt-Lucke ; 2010 Quantification of Circulating Endothelial Progenitor Cells Using the Modified ISHAGE Protocol, November 2010 | Volume 5 | Issue 11 | e13790
Mariuca Vasa, 2001, Number and Migratory Activity of Circulating Endothelial Progenitor Cells Inversely Correlate With Risk Factors for Coronary Artery Disease, Circ Res. 2001;89:e1-e7
Jonathan M. Hill, 2003, Circulating Endothelial Progenitor Cells,Vascular Function, and Cardiovascular Risk N Engl J Med 2003;348:593-600.
Brendan Doyle, 2006 Endothelial Progenitor Cells Endothelium, 13:403–410, 2006 ISSN: 1062-3329 print/1543-5261 online
Christopher J. Boos , 2006 Circulating Endothelial Cells in Cardiovascular Disease, Journal of the American College of Cardiology Vol. 48, No. 8, 2006 ISSN 0735-1097/06
Chambliss KL, Shaul PW. Estrogen modulation of endotelial nitric oxide synthase. Endocr Rev 2002;23:665–686.
Boo YC, Hwang J, Sykes M, : Shear stress stimulates phosphorylation of ENOS at ser(635) by a protein kinase A-dependent mechanism. Am J Physiol Heart CircPhysiol 2002;283:H1819–H1828.
Ziegler T, Silacci P, Harrison VJ, : Nitric oxide synthase expression in endotelial cells exposed to mechanical forces. Hypertension 1998;32:351–355.
Published
2013-10-16
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How to Cite
Nurwidyaningtyas, W., Sargowo, D., Agoes, A., W, T., & Satuman, S. (2013). Ratio of EPC and CEC as Endotel Dysfunction Predictor in High Risk Group with Farmingham Risk Score 10 Years Category. Indonesian Journal of Cardiology, 34(1), 14-9. https://doi.org/10.30701/ijc.v34i1.299
Section
Clinical Research
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